Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations.

摘要: Quantitative, single-voxel proton NMR spectroscopy of normal brain was performed in five adult beagle dogs using the cerebral water signal as an internal intensity reference. The same regions were then rapidly isolated and frozen a pneumatic biopsy drill, perchloric acid extracted, analyzed by biochemical assay high-resolution spectroscopy. concentrations major resonances vivo vitro spectra compared, good agreement found between different measurements. contained three peaks at 3.21, 3.04, 2.02 ppm, which are usually assigned to trimethylamines (TMA), creatines, N-acetyl derivatives (NAc), corresponded be following metabolite concentration values: 1.7 +/- 0.6, 7.7 2.1, 10.9 2.7 mumol/g wet weight respectively. In vitro, measured: glycerophosphocholine (GPC) 1.3 0.2, phosphocholine (PC) 0.5 0.1, phosphocreatine (PCr) 2.6 0.4, creatine (Cr) 5.9 1.4, N-Acetyl aspartate (NAA) 8.9 1.8 weight. Therefore, 3.21 ppm resonance observed spectrum is predominantly GPC PC ratio 2.6:1, 3.04 Cr PCr 2.3:1, (approximately 80%) NAA with small contributions from N-acetylaspartylglutamate (NAAG) glutamate. data presented here validate technique referencing simple convenient means quantitating brain.