A Method for Making Aceto-Carmin Smears Permanent

摘要: Anthers are collected and placed in a solution of 1 part acetic acid to 3 parts absolute alcohol. The contents the anther squeezed out on slide drop Belling's iron-aceto-carmin cover glass over drop. Care should be taken remove all walls flower parts. Heat an alcohol flame for second, repeating 4 or 5 times. Place petri dish filled with 10% acid. When has risen away from gently place Coplin jar containing equal Likewise, this solution. Run both thru following solutions: alcohol, 9 finally xylol. Recombine xylol-balsam directly