Histopathologic and Immunohistochemical Analysis of the Filtration Bleb After Unsuccessful Glaucoma Seton Implantation

摘要: Purpose To analyze histopathologically and immunohistochemically the nitration bleb after unsuccessful glaucoma seton implantation. Methods A von Denffer implant two Molteno implants that were nonfunctional at three months, 11 five years implantation, respectively, compared by evaluating adjacent with light microscopy a panel of antibodies to epithelial, mesenchymal, inflammatory cells. Results The wall filtration months implantation consisted loosely arranged collagenous connective tissue relatively abundant fibroblasts labeled monoclonal (MAbs) V9 Vim 3B4 vimentin. At years, layers had become increasingly thick scarce. All blebs showed metaplastic myofibroblasts surrounded tenascin, as identified M Ab 1 A4 α-smooth muscle actin TN2 respectively. One was lined monolayer cells reacted CAM 5.2 CY-90 cytokeratin 8 18, apparently derived from identically reacting proliferating corneal endothelial Another harbored many macrophages foreign-body giant Little evidence chronic inflammation around detected. Conclusions may induce several processes potentially decrease filtration. In addition formation cyst, presence suggests longstanding scar modulation leads compaction membrane. Furthermore, invade bleb.