Effects of broccoli extract and various essential oils on intestinal and faecal microflora and on xenobiotic enzymes and the antioxidant system of piglets

摘要: Objective: Since the ban of antibiotics as growth promoting feed additives in EU 2006 research alternatives has gained importance. Phytogenic represent a heterogenous class different plant derived substances that are discussed to improve health farm animals by direct and indirect antioxidant effects influencing microbial eubiosis gastrointestinal tract. Consequently our study aimed investigate influence broccoli extract essential oils turmeric, oregano, thyme rosemary, selected individual additives, on intestinal faecal microflora, xenobiotic enzymes, system piglets. Methods: 48 four weeks old male weaned piglets were assigned 6 groups 8. The housed individually stainless steel pens with slatted floor. control group (Con) was fed diet without an additive for 4 weeks. BE contained 0.15 g/kg sulforaphane form extract. 535, 282, 373 476 mg/kg turmeric (Cuo), oregano (Oo), (To) rosemary (Ro) added diets remaining standardise supplementation 150 oils’ key terpene compounds ar-turmerone, carvacrol, thymol 1,8-cineole. composition bacterial microflora examined cultivating samples jejeunal colonic mucosa faeces under specific conditions. mRNA expression enzymes determined reversing transcriptase real time detection PCR (RT-PCR). Total status assayed using Trolox Equivalent Antioxidant Capacity (TEAC), lipid peroxidation measuring thiobarbioturic acid reactive (TBARS). Results: Compared Con all positively influenced weight gain conversion week 1. Over whole trial period no significant differences performance parameters existed between experimental groups. Ro was, however, slightly impaired. Cuo, Oo To increased ratio Lactobacilli:E. coli attached jejunal mucosa, whereas impaired this slightly. In contrast improved ratio. improvement could be analysed Ro. only reduced incidence rate tested positive enterotoxic E. (ETEC). All significantly TEAC TBA-RS. liver BE, tendency significantly. Liver TBA-RS compared Whereas mainly from induction (indirect effects), Cuo effects. Discussion Conclusions: Our results have shown: That within labiatae potential phytogenic small but not microflora.