The effect of equine metabolic syndrome on the ovarian follicular environment.

摘要: Obesity in many species is associated with reduced fertility and increased risk of metabolic disorders cardiovascular dysfunction offspring. Equine syndrome (EMS) obesity characterized by insulin resistance, decreased adiponectin, elevated insulin, leptin, pro-inflammatory cytokines. These alterations can potentially disrupt follicular development impair fertility. We hypothesized that mares EMS have an altered environment when compared to their normal counterparts, affecting gene regulation for follicle oocyte maturation. Samples were collected from light-horse (11 27 yr) a clinical assisted reproductive program. Mares screened based on phenotype. Insulin sensitivity was determined using two proxies, the reciprocal square root (RISQI) modified insulin-to-glucose ratio (MIRG). resistant (RISQI 5.50) allocated group (n = 8), remaining considered controls (CON, n 12). Follicular fluid (FF) granulosa cells (GC) preovulatory follicles aspirated 24 ± 2 h after administration GnRH analog (SucroMate, 0.9 1.4 mg, i.m.) hCG (Chorion, 1500 2000 IU, i.v.). After overnight fast, blood morning aspiration evaluate serum concentrations inflammatory Expression 32 genes related metabolism, maturation, maturation assessed GC. Concentrations cytokines highly correlated between FF (P < 0.001). lower 0.001) CON EMS, but leptin IL1β tended 0.07 P 0.10, respectively) be than EMS. Tumor necrosis factor-α 0.05, Conversely, adiponectin higher 0.05) versus In GC expression epiregulin tissue inhibitor matrix metalloproteinase-2 0.09). Our findings demonstrate intrafollicular mare influenced disease, consistent other species. Influences development, subsequent offspring perturbations due disease need further study.