Vector-based RNA interference against vascular endothelial growth factor-A significantly limits vascularization and growth of prostate cancer in vivo.

摘要: RNA interference technology is emerging as a very potent tool to obtain cellular knockdown of desired gene. In this work we used vector-based inhibit vascular endothelial growth factor (VEGF) expression in prostate cancer vitro and vivo. We demonstrated that transduction with plasmid carrying small interfering targeting all isoforms VEGF, dramatically impairs the human cell line PC3. As consequence, PC3 cells loose their ability induce one fundamental steps angiogenesis, namely formation tube-like network vitro. Most importantly, our "therapeutic" vector able impair tumor rate vascularization show single injection naked developing neoplastic mass significantly decreases microvessel density an androgen-refractory xenograft sustain long-term slowing down growth. conclusion, results confirm basic role VEGF angiogenic development carcinoma, suggest use approach angiogenesis could be effective view future gene therapy applications for cancer.