Monoclonal antibodies specific for the tau subunit of the DNA polymerase III holoenzyme of Escherichia coli. Use to demonstrate that tau is the product of the dnaZX gene and that both it and gamma, the dnaZ gene product, are integral components of the same enzyme assembly.

摘要: Abstract We have established two murine hybridoma cell lines that secrete monoclonal antibodies directed against the tau subunit of DNA polymerase III holoenzyme Escherichia coli. Both been purified and identified to be IgG1 class. Competition assays indicate they bind distinct portions subunit. These used demonstrate is an integral part all assemblies product dnaZX gene. react only with tau, not gamma, other protein Immunoprecipitation studies demonstrated contained within same enzyme as gamma (dnaZ protein). This observation discussed in light functioning asymmetric dimer, capable coordinating leading lagging strand replication.