Zwitterionic-hydrophilic interaction capillary liquid chromatography coupled to tandem mass spectrometry for the characterization of human alpha-acid-glycoprotein N-glycan isomers

摘要: Abstract In this work, a μZIC-HILIC-MS/MS methodology was established in negative ion mode for the characterization of glycan isomers. The possibility to separate isomers by μZIC-HILIC strategy coupled high resolution tandem mass spectrometry detection permitted us obtain valuable information about each structure. most important diagnostic fragments previously described characterize structural features glycans, were evaluated study using hAGP as model glycoprotein. assignation performed our previous work GRIL combination with exoglycosidase digestion [1] used paper confirm or discard some reported literature and delve into Sialic acid well fucose linkage-type assigned approach daughter ions higher value determined. location α2-3/α2-6 sialic acids on antennas deeper several highly sialylated tri- tetraantennary glycans also possible MS/MS method. Moreover, relying Ref. [1], core antenna fucosylation differentiated specific obtained spectra. This applied purified from control pathological serum samples, which corroborated its robustness potential finding novel glycan-based biomarkers patho-glycomic studies.