Isolation of new phosphorylated glycoprotein from mineralized phase of bone that exhibits limited homology to adhesive protein osteopontin.
作者： J P Gorski , K Shimizu
DOI: 10.1016/S0021-9258(18)37539-2
关键词:
摘要: Extracts of the mineralized phase rat calvaria were shown to contain bone acidic glycoprotein-75, a new phosphorylated glycoprotein which co-purifies with small proteoglycans through anion-exchange chromatography. Final purification each was brought about subsequent hydroxyapatite step. Bone glycoprotein-75 is 75,000 in molecular weight 29.3% molar content amino acid residues, 7.0% (w/w) sialic acid, and 7.9% organic phosphate. Its N-terminal sequence determined as Leu-Pro-Val-Ala-Arg-Tyr-Gln-Asn-Thr-Glu-Glu-Glu-Glu-. Because size charge density properties are similar those reported for sialoprotein II, calvarial II also purified homogeneity, its composition determined. The results showed an identity first 5 residues human complete lack homology which, furthermore, did not bind anti-sialoprotein antibodies. Although appears be unique, 33% shared adhesive protein osteopontin. Affinity-purified antibodies against osteopontin found specifically upon immunoblotting, whether proteins or components crude extracts. In summary, from compartment tissue limited structural
sci-hub.st 参考文章(44)
L.A. Fransson, I Sjöberg, V.P. Chiarugi, Co-polymeric glycosaminoglycans in transformed cells. Transformation-dependent changes in the self-associating properties of cell-surface heparan sulfate. Journal of Biological Chemistry. ,vol. 256, pp. 13044- 13047 ,(1981) , 10.1016/S0021-9258(18)43003-7
I.J. Mason, A. Taylor, J.G. Williams, H. Sage, B.L. Hogan, Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell culture shock glycoprotein of Mr 43 000 The EMBO Journal. ,vol. 5, pp. 1465- 1472 ,(1986) , 10.1002/J.1460-2075.1986.TB04383.X
Paul A. Price, Vitamin K-Dependent Formation of Bone Gla Protein (Osteocalcin) and Its Function Vitamins and Hormones Series. ,vol. 42, pp. 65- 108 ,(1985) , 10.1016/S0083-6729(08)60061-8
L W Fisher, S W Whitson, L V Avioli, J D Termine, Matrix sialoprotein of developing bone. Journal of Biological Chemistry. ,vol. 258, pp. 12723- 12727 ,(1983) , 10.1016/S0021-9258(17)44236-0
J.E. Christner, B. Caterson, J.R. Baker, Immunological determinants of proteoglycans. Antibodies against the unsaturated oligosaccharide products of chondroitinase ABC-digested cartilage proteoglycans. Journal of Biological Chemistry. ,vol. 255, pp. 7102- 7105 ,(1980) , 10.1016/S0021-9258(20)79670-5
J.D. Termine, A.B. Belcourt, P.J. Christner, K.M. Conn, M.U. Nylen, Properties of dissociatively extracted fetal tooth matrix proteins. I. Principal molecular species in developing bovine enamel. Journal of Biological Chemistry. ,vol. 255, pp. 9760- 9768 ,(1980) , 10.1016/S0021-9258(18)43458-8
Grant R. Bartlett, Phosphorus Assay in Column Chromatography Journal of Biological Chemistry. ,vol. 234, pp. 466- 468 ,(1959) , 10.1016/S0021-9258(18)70226-3
C.W. Prince, T. Oosawa, W.T. Butler, M. Tomana, A.S. Bhown, M. Bhown, R.E. Schrohenloher, Isolation, characterization, and biosynthesis of a phosphorylated glycoprotein from rat bone. Journal of Biological Chemistry. ,vol. 262, pp. 2900- 2907 ,(1987) , 10.1016/S0021-9258(18)61592-3
J.W. Poser, P.A. Price, A method for decarboxylation of gamma-carboxyglutamic acid in proteins. Properties of the decarboxylated gamma-carboxyglutamic acid protein from calf bone. Journal of Biological Chemistry. ,vol. 254, pp. 431- 436 ,(1979) , 10.1016/S0021-9258(17)37935-8
L W Fisher, G R Hawkins, N Tuross, J D Termine, Purification and partial characterization of small proteoglycans I and II, bone sialoproteins I and II, and osteonectin from the mineral compartment of developing human bone. Journal of Biological Chemistry. ,vol. 262, pp. 9702- 9708 ,(1987) , 10.1016/S0021-9258(18)47991-4