Precision Mapping of an In Vivo N-Glycoproteome Reveals Rigid Topological and Sequence Constraints
作者： Dorota F Zielinska , Florian Gnad , Jacek R Wiśniewski , Matthias Mann , None
DOI: 10.1016/J.CELL.2010.04.012
关键词:
摘要: N-linked glycosylation is a biologically important protein modification, but only a small fraction of modification sites have been mapped. We developed a “filter aided sample preparation”(FASP)-based method in which glycopeptides are enriched by binding to lectins on the top of a filter and mapped 6367 N-glycosylation sites on 2352 proteins in four mouse tissues and blood plasma using high-accuracy mass spectrometry. We found 74% of known mouse N-glycosites and discovered an additional 5753 sites on a diverse range of proteins …
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