Blood clearance and tissue uptake of intravenous lipid emulsions containing long-chain and medium-chain triglycerides and fish oil in a mouse model.

摘要: BACKGROUND Increasing interest in using different triglycerides (TGs) for specific clinical applications raised the question as to how emulsion TG composition would affect blood clearance and delivery hepatic extrahepatic tissues. METHODS Emulsions used were long-chain soy oil (long-chain triglyceride [LCT]), LCT/ medium-chain (MCT; 1:1, wt/wt), LCT/MCT/C/omega-3 (5:4:1, wt/wt) pure fish (omega-3 TG) labeled with non-degradable 3H-cholesteryl oleoyl ether (3H-CE) a particle marker. Mice (C57BL/6J) injected four commercial emulsions at nonsaturating dose of 0.4 mg TG/20 25 g per mouse obtain 1st order kinetics. Blood was sampled 0.5, 2, 5, 10, 15, minutes, fractional catabolic rate determined by fitting straight line logarithm 3H-CE radioactivity. Retention each tissue minutes reflected organ uptake emulsion. RESULTS omega-3 (10.40% +/- 0.54% pools/h; mean SE) significantly slower than that LCT, LCT/MCT, LCT/MCT/omega-3 (18.9 0.6 pools/h, 17.0 0.96 16.5 1.08 respectively) (p < .01). Based on uptake, showed similar liver (39% 3.9%, 46% 3.6%, 34% 3.2%). Liver LCT/MCT/omega-3, (23% 2.2%) less LCT/MCT (46% +/-3.6%, p .0001) LCT = .002). CONCLUSIONS Results indicate slow from compared which mixed MCT. Earlier data are poorly hydrolyzed extracellular media therefore delivered tissues part core remnants. Thus, our suggest incorporation will result greater fatty acids tissue, could be important modulating immune other responses.