Anti-inflammatory and cytoprotective effects of selected Pakistani medicinal plants in Helicobacter pylori-infected gastric epithelial cells.

摘要: Abstract Ethnopharmacological relevance Helicobacter pylori infection is associated with gastritis, peptic ulcer, and gastric cancer. Due to its high global prevalence uprising resistance available antibiotics, efforts are now directed identify alternative source treat prevent disorders. In the present study, effect of selected indigenous medicinal plants Pakistan was evaluated on secretion interleukin-8 (IL-8) generation reactive oxygen species (ROS) in a bid rationalize their use examine anti-inflammatory cytoprotective effects epithelial cells. Materials methods AGS cells clinically isolated strain (193C) were employed for co-culture experiments. Anti-Helicobacter activity cytotoxic determined by serial dilution method DNA fragmentation assay respectively. ELISA flow cytometry performed evaluate IL-8 ROS pylori-infected Results At 100 μg/ml, extracts Alpinia galangal, Cinnamomum cassia, tamala, Mentha arvensis, Myrtus communis, Oligochaeta ramose, Polygonum bistorta, Rosa damascena, Ruta graveolens, Syzygium aromaticum, Tamarix dioica, Terminalia chebula exhibited strong inhibitory against secretion. Of these, four markedly inhibited at both 50 100 μg/ml. cassia further assessed different concentrations TNF-α stimulated secretion, which displayed significant suppression concentration-dependent-manner. Among examined generation, Achillea millefolium, Berberis aristata, Coriandrum sativum, Foeniculum vulgare, Matricaria chamomilla Prunus domestica demonstrated from (p  Conclusion study revealed could partially validate traditional these GI disorders particularly pylori. Furthermore, results obtained may lead possible future candidates chemoprevention ulcer or