Genetic variability in proso millet [ Panicum miliaceum ] germplasm of Central Himalayan Region based on morpho-physiological traits and molecular markers

摘要: Sixteen representative a ccessions of proso millet [Panicum miliaceum] having distinct traits agronomic importance were collected from altitudinal range 510–2,695 m asl in the Central Himalayan Region (CHR) India. Considerable diversity was found morpho-physiological viz., leaf length (16.80–32.00 cm), width (1.7–2.1 cm), plant height (57.00–134.00 cm), days to 50 % flowering (34–54), 80 % maturity (111–144), and 1,000 seed weight (0.68–1.86 g). Collected accessions have been evaluated for a battery biochemical parameters chlorophyll, carotenoids, lipid peroxidation, cellular hydrogen peroxide, activity nitrate reductase, lipoxygenase, catalase, peroxidase, superoxide dismutase along with super oxide free radical formation, glutathione (total, reduced, oxidized), S-transferase, ascorbate monodehydroascorbate didehydroascorbate reductase. The sets 12 genic-SSRs (simple sequence repeats), 54 ISSR (inter simple 40 SRAP (sequence related amplified polymorphism) markers used study level genetic diversity, Nei’s gene value 0.20 obtained both markers. showed higher average number polymorphic bands, % polymorphism, information content (PIC), Shannon index compared markers; no allelic variation. Cluster analysis shows close groupings germplasm based on as well molecular diverse identified considerable may be utilized development climate resilient cultivars.