Alternative inactivated poliovirus vaccines adjuvanted with Quillaja brasiliensis or Quil-a saponins are equally effective in inducing specific immune responses.

摘要: Inactivated polio vaccines (IPV) have an important role at the final stages of poliomyelitis eradication programs, reducing risks associated with use attenuated vaccine (OPV). An affordable option to enhance immunogenicity and reduce costs IPV may be effective renewable adjuvant. In present study, adjuvant activity aqueous extract (AE) saponin fraction QB-90 from Quillaja brasiliensis using poliovirus antigen as model were analyzed compared a preparation adjuvanted Quil-A, well-known saponin-based commercial Experimental prepared viral plus saline (control), Quil-A (50 µg), AE (400 µg) or µg). Sera inoculated mice collected days 0, 28, 42 56 post-inoculation first dose vaccine. Serum levels specific IgG, IgG1 IgG2a significantly enhanced by AE, control group on day 56. The magnitude enhancement was statistically equivalent for Quil-A. cellular response evaluated through DTH analysis IFN-γ IL-2 mRNA in vitro reestimulated splenocytes. Results indicated that capable stimulating generation Th1 cells against administered same extent Mucosal immune demonstrated increases IgA titers bile, feces vaginal washings, yielding comparable higher than results obtained indicate saponins Q. are potent adjuvants humoral responses represent viable