Cationic Synthetic Peptides: Assessment of Their Antimicrobial Potency in Liquid Preserved Boar Semen

作者: Stephanie Speck , Alexandre Courtiol , Christof Junkes , Margitta Dathe , Karin Müller

DOI: 10.1371/JOURNAL.PONE.0105949

关键词:

摘要: Various semen extender formulas are in use to maintain sperm longevity and quality whilst acting against bacterial contamination liquid preservation. Aminoglycosides commonly supplemented aid the control of bacteria. As resistance is increasing worldwide, antimicrobial peptides (AMPs) received lively interest as alternatives overcome multi-drug resistant We investigated, whether synthetic cationic AMPs might be a suitable alternative for conventional antibiotics boar The antibacterial activity two cyclic (c-WWW, c-WFW) helical magainin II amide analog (MK5E) was studied vitro Gram-positive eleven Gram-negative Isolates included ATCC reference strains, multi-resistant E. coli bacteria cultured from semen. Using broth microdilution, minimum inhibitory concentrations were determined all AMPs. All revealed towards majority but not Proteus spp. (all AMPs) Staphylococcus aureus 29213 (MK5E). could also demonstrate that c-WWW c-WFW effective growth preserved situ, especially when combined with small amount gentamicin. Our results suggest albeit offering complete traditional antibiotics, offers promising solution decrease thereby limit selection strains.

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