Uncovering the genetic architecture of Colletotrichum lindemuthianum resistance through QTL mapping and epistatic interaction analysis in common bean.

摘要: Colletotrichum lindemuthianum is a hemibiotrophic fungal pathogen that causes anthracnose disease in common bean. Despite the genetics of resistance has been studied for long time, few quantitative trait loci (QTLs) studies have conducted on this species. The present work examines genetic basis to races 23 and 1545 C. different organs (stem, leaf petiole). A population 185 recombinant inbred lines (RIL) derived from cross PMB0225 x PHA1037 was evaluated under natural artificial photoperiod growth conditions. Using multi-environment QTL mapping approach, 10 16 main effect QTLs were identified 1545, respectively. homologous genomic regions corresponding 17 26 detected positive presence resistance-associated gene cluster encoding nucleotide-binding leucine-rich repeat (NL) proteins. Among them, it worth noting linkage group 05 race stem, petiole located within 1.2 Mb region. NL Phvul.005G117900 region, which can be considered an important candidate non-organ-specific here. Furthermore, total 39 epistatic (E-QTLs) (21 18 1545) involved 20 interactions (eleven nine respectively) identified. None displayed significant environment interaction effects. research provides essential information not only better understanding plant-pathogen but also application assisted breeding improvement bean through marker-assisted selection (MAS).