Rapid plant regeneration in industrially important Curcuma zedoaria revealing genetic and biochemical fidelity of the regenerants

摘要: The present investigation was carried out to establish an efficient and reproducible micropropagation protocol for the production of morphologically, genetically chemically uniform plants Curcuma zedoaria. Axillary bud explants C. zedoaria were inoculated into MS basal medium supplemented with various combinations concentrations 6-benzyladenine (2.2–22.2 µM, BA), kinetin (2.3–23.2 µM, Kin), indole-3-acetic acid (2.9–11.4 µM, IAA), α-naphthalene acetic (2.7–10.2 µM, NAA) adenine sulphate (33.9–203.6 µM, Ads). Almost 95% rhizome buds sprouted on 13.3 μM BA, 5.7 μM IAA 63.9 μM Ads giving rise average 12.89 ± 0.02 shoots within 6 weeks. However, maximum number roots (25.8 ± 0.07 per explant) obtained half strength 7.4 µM IBA after 4 weeks inoculation. Morphological characteristics similar in both conventionally propagated micropropagated plants. Additionally, genetic homogeneity vitro further confirmed through ISSR flow cytometry analysis. A total 27 primers screened, which 13 generated 58 monomorphic bands thereby confirming uniformity mean 2C DNA content mother plant (2.96 pg) that derived (3.07 pg). Gas chromatography-mass spectrometry (GC–MS) analysis showed similarity qualitative profile chemical constituents essential oil high-performance liquid chromatography revealed no significant differences curcumin tissue culture regenerants Therefore, could be effectively employed generate true type plantlets