A rapid boiling method for the preparation of bacterial plasmids

作者: David S. Holmes , Michael Quigley

DOI: 10.1016/0003-2697(81)90473-5

关键词:

摘要: … lysate procedure (1). We have not examined the relationship between plasmid yield and the components of the boiling … is to weaken the bacterial cell wall even though the reaction must …

参考文章(10)
Bernhard Hirt, Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology. ,vol. 26, pp. 365- 369 ,(1967) , 10.1016/0022-2836(67)90307-5
Michael Zasloff, Gordon D. Ginder, Gary Felsenfeld, A new method for the purification and identification of covalently closed circular DNA molecules Nucleic Acids Research. ,vol. 5, pp. 1139- 1152 ,(1978) , 10.1093/NAR/5.4.1139
H.C. Birnboim, J. Doly, A rapid alkaline extraction procedure for screening recombinant plasmid DNA Nucleic Acids Research. ,vol. 7, pp. 1513- 1523 ,(1979) , 10.1093/NAR/7.6.1513
Thomas C. Currier, Eugene W. Nester, Isolation of Covalently Closed Circular DNA of High Molecular Weight From Bacteria Analytical Biochemistry. ,vol. 76, pp. 431- 441 ,(1976) , 10.1016/0003-2697(76)90338-9
Francisco Bolivar, Raymond L. Rodriguez, Patricia J. Greene, Mary C. Betlach, Herbert L. Heyneker, Herbert W. Boyer, Jorge H. Crosa, Stanley Falkow, Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. ,vol. 2, pp. 95- 113 ,(1977) , 10.1016/0378-1119(77)90000-2
Alan COLMAN, Michael J. BYERS, Sandy B. PRIMROSE, Alan LYONS, Rapid Purification of Plasmid DNAs by Hydroxyapatite Chromatography FEBS Journal. ,vol. 91, pp. 303- 310 ,(1978) , 10.1111/J.1432-1033.1978.TB20966.X