A proteomic analysis of Klebsiella oxytoca after exposure to succinonitrile

摘要: Abstract Succinonitrile, an environmental pollutant, has been proved to be degraded by Klebsiella oxytoca. However, the growth rate of this bacterium was decreased succinonitrile. The aim study is determine effects succinonitrile on physiological responses K. oxytoca were investigated. Using two-dimensional polyacrylamide gel electrophoresis (2-DE) and MALDI-TOF-MS, we identified 7 differentially expressed proteins in treated with comparison cells ammonia. expression one protein, ribosomal protein L9, involved biosynthesis pathway functional ribosome. increased such as superoxide dismutase (SOD), glutathione-S-transferase (GST), PsaA, a metal binding might detoxification process succinonitrile, which suggested that biodegradation accompanied occurrence oxidative stress. Thus, stress plays major role toxicity vivo, cellular defense against involves activation antioxidant proteins. Although limited compound, minimizing during bolstering capacity deal will improve their effectiveness biodegradation. With help proteomic analysis, can understand about toxic mechanism and/or gain more information deeper understanding