A high-performance DNA biosensor based on the assembly of gold nanoparticles on the terminal of hairpin-structured probe DNA
作者： Songling Li , Weiwei Qiu , Xuan Zhang , Jiancong Ni , Feng Gao
DOI: 10.1016/J.SNB.2015.09.121
关键词:
摘要: Abstract An ultrasensitive electrochemical DNA biosensor has been developed based on the assembly of highly conductive gold nanoparticles (AuNPs) free terminal hairpin-structured probe DNA. The hairpin was first immobilized a electrode surface through Au S bond. Then thioglycolic acid (TGA) grafted 3′-end via carboxylic-amino condensation reaction. Followed by, AuNPs were further attached reaction with thiol-group TGA. Thus, biointerface ultralow charge-transfer resistance obtained. Quantitative analysis exhibited that target could be detected over range from 1.0 × 10−17 to 1.0 × 10−11 M. detection limit estimated 1.7 × 10−18 M. Also showed good hybridization selectivity, stability and regeneration ability.
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