Nitric oxide enhances Oct-4 expression in bone marrow stem cells and promotes endothelial differentiation.
作者： Ling Chu , Yuehua Jiang , Hong Hao , Yong Xia , Jian Xu
DOI: 10.1016/J.EJPHAR.2008.06.066
关键词:
摘要: This study was designed to investigate the role of nitric oxide (NO) in bone marrow stem cells and their differentiation into endothelial vitro. Adult mouse multipotent progenitor (MAPCs) were used as source cells. Oct-4 expression (both mRNA protein) significantly increased by up 68.0% MAPCs when incubated with NO donors DETA-NONOate or sodium nitroprusside (SNP) a concentration-dependant manner (n=3, P<0.05). However, cell proliferation dramatically decreased over 3-folds treated SNP for 48 h When exposed (100 microM) first during differentiation, vWF at day 14 differentiating The effects on proliferation, not affected guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one cGMP analog 8-Br-cGMP. These data indicate that may regulate both pluripotency via cGMP-independent mechanism.
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