Isolation, characterization and comparative analysis of plant-associated bacteria for suppression of soil-borne diseases of field-grown groundnut in Vietnam

摘要: Abstract Groundnut (Arachis hypogaea L.) is an important oil seed crop worldwide and used extensively for feed food. In Vietnam, groundnut cultivation hampered by several soil-borne fungal pathogens, in particular Sclerotium rolfsii. To develop sustainable measures to control stem rot disease caused S. rolfsii, plant-associated bacteria were isolated from the base roots of plants grown farmer fields central Vietnam tested activity against Among a total 3,360 randomly selected bacterial isolates, only thirteen (0.4%) inhibited hyphal growth BOX-PCR 16S-rDNA sequence analyses revealed that these isolates genetically diverse belonged three Phyla, i.e. γ-Proteobacteria (Pseudomonas), Firmicutes (Bacillus) Bacteroidetes (Chryseobacterium). Nethouse field experiments conducted showed treatment seeds or soil with strains each genera significantly reduced incidence disease, led significant yield increases up 21% did not have adverse effects on nodulation. The level protection provided was similar achieved fungicide tebuconazole. Comparative analysis biocontrol efficacy indigenous Pseudomonas strain R4D2 two exogenous, antagonistic Netherlands trials better colonized groundnut, (S. rolfsii), black collar (Aspergillus niger), wilt (Ralstonia solanacearum), more consistently enhanced yield.