Immunocytochemical demonstration of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in human brain tumor tissues.

摘要: Five patients with various brain tumors received bromodeoxyuridine (BrdU), 150–200 mg/m2 i.v.; at the time of craniotomy. Biopsied materials were fixed in 70% ethanol, sectioned, denatured hydrochloric acid, and reacted monoclonal antibodies against BrdU. Immunofluorescence immunocytochemical methods used to visualize BrdU-labeled nuclei. Our results showed that both demonstrated nuclei satisfactorily tissue sections. Thus, BrdU can be measure proliferative potential human situ.