[22] Receptor-mediated endocytosis in semiintact cells

摘要: Publisher Summary This chapter discusses receptor-mediated endocytosis in semiintact cells. Many biologically important macromolecules are internalized into cells with the complex, multistep process of endocytosis. Bound ligands and their receptors first clustered specialized coated pit regions cell surface. Cell surface via pits fall two classes. One class, including, transferrin low-density lipoprotein (LDL) receptors, constitutively localized efficiently at rates independent bound ligand. In contrast, unoccupied such as epidermal growth factor (EGF) insulin low basal rates. These events not only require addition coat constituents but accompanied by morphological changes coat, which progresses from an initially planar structure to a highly curved, invaginated pit. Finally, membrane fission event occurs is sealed around newly budded vesicle. Molecular dissection these biochemically diverse facilitated use cell-free assays that allow distinct stages be assayed independently. chapter, assay described was used examine A431 measurement assembly, invagination, vesicle budding.