Biohydrogen production via biocatalyzed electrolysis in acetate-fed bioelectrochemical cells and microbial community analysis

摘要: Abstract Hydrogen was efficiently produced via acetate oxidation using a two-chambered bioelectrochemical cell (BEC), modified microbial fuel (MFC), in which cathode kept free of oxygen and the thermodynamic barrier overcome by augmenting electrochemical potential achieved bacteria with addition small external voltage. The production hydrogen gradually increased increasing applied voltage from 0.1 to 1.0 V, reaching its maximum efficiency 52.5% at 0.8 V, corresponding yield 2.1 mol per 1 mol acetate. anodic loss electrons more detrimental compared that cathodic loss, especially higher range. headspace gas consisted mainly (>96.6%), but minor methane (2.5%) carbon dioxide (0.9%) impurities diffused anode chamber. To maintain purity or prevent produced, much concern is required for control CO2 CH4 because these gases diffuse readily through Nafion 117 membrane than H2. produce hydrogen, continuous augmentation circuit an had no harmful effect on bacterial viability resulted remarkable change community. There substantial decrease species diversity single emergent Pelobacter propionicus-like BEC. Interestingly, Geobacter-like were integral members consortia MFC