PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screening.

摘要: Precise monitoring of specific biomarkers in biological fluids with accurate biodiagnostic sensors is critical for early diagnosis diseases and subsequent treatment planning. In this work, we demonstrated an innovative sensor, portable reusable diagnostics nanostar antennas (PRADA), multiplexed biomarker detection small volumes (~50 μl) enabled a microfluidic platform. Here, PRADA simultaneously detected two myocardial infarction, cardiac troponin I (cTnI), which well accepted disorders, neuropeptide Y (NPY), controls sympathetic drive. immunoassay, magnetic beads captured the human serum samples, gold nanostars (GNSs) "antennas" labeled peptide biorecognition elements Raman tags via surface-enhanced spectroscopy (SERS). The peptide-conjugated GNS-SERS barcodes were leveraged to achieve high sensitivity, limit (LOD) 0.0055 ng/ml cTnI, LOD 0.12 ng/ml NPY comparable commercially available test kits. innovation was also regeneration reuse same sensor chip ~14 cycles. We validated by testing cTnI 11 de-identified patient samples various demographics within 95% confidence interval precision profile. envision low-cost will have tremendous translational impact be amenable resource-limited settings planning patients.