Analysis of the cellular infiltrate in the iris during experimental autoimmune encephalomyelitis.

摘要: PURPOSE. Previous studies have shown that experimental autoimmune encephalomyelitis (EAE) and anterior uveitis (AU) develop in Lewis rats immunized with myelin basic protein (MBP). The purpose of this study was to characterize the dynamics, distribution, phenotype infiltrating cells iris during EAE-associated AU. METHODS. were MBP emulsified complete Freund's adjuvant (CFA) or CFA alone. Cellular infiltration analyzed at various time points by immunohistochemistry wholemounts, flow cytometry, immunoelectron microscopy, using monoclonal antibodies specific for monocytes/macrophages (ED1), T lymphocytes (R73, W3.25, OX8), T-cell activation markers (0X39, 0X40), granulocytes (HIS48), major histocompatibility complex (MHC) class II (0X6), neurofilament (2H3). RESULTS. MBP-immunized showed development characteristic monophasic EAE, followed, after resolution paralysis, mild self-limited Initially, focal infiltrates round MHC + ED1 found iris. During course AU, midiris became massively infiltrated monocytes-macrophages, R73 cells, (HIS48 ). cells. influx consisted CD4 CD8 which only a small fraction (<14 11%, respectively) expressed markers. accumulated proximity myelinated nonmyelinated nerve bundles vicinity blood vessels No evidence demyelination degradation. Neither EAE nor AU developed CFA-treated control rats. CONCLUSIONS. These data show is characterized transient mixed cellular infiltrate consisting granulocytes, preferential accumulation inflammatory fibers suggests model may result from autoreactivity antigens.