Antibodies as Probes of G-Protein Structure and Function
作者： Allen M. Spiegel , William F. Simonds , Paul K. Goldsmith , Cecilia G. Unson
DOI: 10.1007/978-1-4613-0559-0_12
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摘要: G-proteins involved in signal transduction are members of a guanine nucleotide-binding protein superfamily that includes cytoskeletal proteins such as tubulin, soluble (initiation and elongation factors synthesis), low molecular weight GTP-binding the ras p21 protooncogenes ras-related (Spiegel, 1987; Gilman, Iyengar & Birnbaumer, 1987). Members G-protein subset share certain general features common with other superfamily: 1) all bind nucleotides high affinity specificity, possess intrinsic GTPase activity modulates interactions between elements; 2) serve substrates for ADP-ribosylation by bacterial toxins; this covalent modification disrupts normal function.
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