Detection of cytotoxic T lymphocytes specific for synthetic peptides of gp160 in HIV-seropositive individuals.

摘要: Four synthetic peptides corresponding to the IIIB sequence of gp160 HIV were recently reported stimulate Th cell function by PBL from HIV-infected, asymptomatic patients. In present report, we used these same demonstrate CTL activity in a similar patient population. EBV-transformed B-cell lines asymptomatic, seropositive and seronegative control donors pre-incubated with peptides. Fresh 19 (76%) 25 lysed autologous targets pulsed at least one four Autologous two non-immunogenic not lysed. none eight controls peptide-preincubated targets. The was mediated T cells, predominantly MHC class I restricted, increased vitro restimulation HLA A-2 identified as restricting element for all different patients, three donor. HLA-A1 or -B8 may also some Thus, can be recognized human cells MHC-restricted CTL.