Genetic relationships among accessions of mandacaru (Cereus spp.: Cactaceae) using amplified fragment length polymorphisms (AFLP)
作者： Juliana Sala de Faria-Tavares , Paula Garcia Martin , Claudete Aparecida Mangolin , Sandra Aparecida de Oliveira-Collet , PS Maria de Fátima
DOI: 10.1016/J.BSE.2012.11.013
关键词:
摘要: Amplified fragment length polymorphism (AFLP) analysis was used to evaluate the genetic diversity at DNA level of mandacaru (genus Cereus) and differentiate between 17 accessions grown in different regions Brazil. The six primer pairs amplified 348 AFLP markers, which 282 (81%) were polymorphic. percentage polymorphic fragments ranged from 62.5% for combination E-AAC × M-CAG 91.7% E-ACT M-CAC. largest number informative markers (67) detected using E-ACA M-CAG, while E-AGC M-CTC revealed lowest (46) plantlets. Nei's identity value 0.6348–0.8343 plants Southern Northeastern regions, 0.6348–0.6529 Southwestern 0.6193–0.6944 regions. similarity among Southern, Northeastern, indicates that three may be species Cereus genus: peruvianus or repandus (Southern region), jamacaru (Northeastern hildmaniannus (Southwestern region). Alternatively, belong one process speciation.
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