Alternative nutrient sources for biotechnological use of Sporosarcina pasteurii.

摘要: The potential use of Sporosarcina pasteurii in possible biotechnological applications on a large scale (ground improvement, consolidation building structures and ornamental stone, or developing bio-materials for the industry), is based its ability to produce high amounts carbonate short period time via urea hydrolysis. Industrial biomass production would have low environmental impact be most economical if standard growth media could replaced with alternative nutrient sources, such as byproducts wastes from other industries, cost ingredients. effective ingredients must guarantee ureolytic activities conditions that are comparable those resulting medium. In this work, three types were tested growing active bacteria S. pasteurii: (1) sources industrial dairy brewery (2) fertilizer an substitute, (3) different poultry manure fertilizers substitutes. comparison between media, alternatives substitutes was by taking protein concentration nitrogen content into account. Bacterial activity evaluated terms changes over (CFU, optical density, ATP measurements) indirect estimation enzyme (Nessler assay, conductivity measurement). results revealed some tested, whey buttermilk, nutrients bacterial development, while perfectly suitable substitute pure laboratory grade urea.