Characterizing the malignancy and drug resistance of cancer cells from their membrane resealing response.

摘要: In this report, we showed that two tumor cell characteristics, namely the malignancy and drug-resistance status can be evaluated by their membrane resealing response. Specifically, pores in a number of pairs cancer normal lines originated from nasopharynx, lung intestine were introduced nano-mechanical puncturing. Interestingly, such nanometer-sized holes cells reseal ~2–3 times faster than those corresponding cells. Furthermore, time exhibiting resistance to several leading chemotherapeutic drugs was also found substantially shorter drug-sensitive counterparts, demonstrating potential using quantity as novel marker for future diagnosis drug detection. Finally, simple model proposed explain observed dynamics which suggested distinct response exhibited normal, resistant is likely due different tension levels lipid membranes, conclusion supported direct cortical measurement.