Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses

摘要: The use of a complete and integrated in vitro pollination, fertilization embryo rescue system lily was examined. By combining pollination techniques to overcome pre-fertilization barriers with methods post-fertilization barriers, interspecific crosses could be made more efficiently. In cut-style grafted style technique were developed applied on various using Lilium longiflorum, L. dauricum, henryi, both Asiatic Oriental hybrids as the parents. addition, for ovary culture, ovary-slice culture ovule generated. Ovule swelling score used evaluate media effects development. Using protocol it not only possible raise total number hybrid plantlets single cross, but also successful combinations.