Gene Expression Profiling of the Early Pulmonary Response to Hyperoxia in Mice

摘要: To identify molecular events occurring during the early response to hyperoxia, we measured changes over time in total lung gene expression C57BL/6 mice prolonged exposure > 95% O2. Specifically, differential of 8,734 sequence-verified murine complementary DNAs was analyzed after 0, 8, 24, and 48 h O2 exposure, with additional genes interest at 24 h. Of 385 differentially expressed, hyperoxia increased 175 (2.0%) decreased 210 (2.3%). The majority “classic” antioxidant enzymes, including catalase, MnSOD, Cu-Zn SOD, showed no change a number other glutathione peroxidase, glutathione-S-Transferase (GST) Π1, GST μ2, heme oxygenase-1 showing relatively moderate increases. exception heavy metal–binding protein metallothionein, which 7-fold We found o...