High-throughput fluorescence-based isolation of live C. elegans larvae
作者： Anita G Fernandez , Bastiaan O R Bargmann , Emily K Mis , Mark L Edgley , Kenneth D Birnbaum
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摘要: For the nematode Caenorhabditis elegans, automated selection of animals specific genotypes from a mixed pool has become essential for genetic interaction or chemical screens. To date, such been accomplished using specialized instruments. However, access to dedicated equipment is not common. Here we describe live animal fluorescence-activated cell sorting (laFACS), protocol automatic first larval stage (L1) standard FACS system. We show that can be used precise identification GFP-expressing and non-GFP-expressing subpopulations accomplish high-speed animals. have routinely collected 100,000 more homozygotes starting population within 2 h, with greater than 99% purity. The sorted continue develop normally, making this ideally suited isolation terminal mutants use in
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