Protein production: feeding the crystallographers and NMR spectroscopists.
作者： Aled M. Edwards , Cheryl H. Arrowsmith , Dinesh Christendat , Akil Dharamsi , James D. Friesen
DOI: 10.1038/80751
关键词:
摘要: Protein purification efforts for structural genomics will focus on automation the readily-expressed proteins, and process development more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in next few years. The purified valuable reagents entire research community.
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sci-hub.se 参考文章(13)
W.A. Hendrickson, J.R. Horton, D.M. LeMaster, Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD) : a vehicle for direct determination of three-dimensional structure The EMBO Journal. ,vol. 9, pp. 1665- 1672 ,(1990) , 10.1002/J.1460-2075.1990.TB08287.X
Geoffrey S. Waldo, Blake M. Standish, Joel Berendzen, Thomas C. Terwilliger, Rapid protein-folding assay using green fluorescent protein. Nature Biotechnology. ,vol. 17, pp. 691- 695 ,(1999) , 10.1038/10904
Aled M. Edwards, Cheryl H. Arrowsmith, Dinesh Christendat, Adelinda Yee, Akil Dharamsi, Yuval Kluger, Alexei Savchenko, John R. Cort, Valerie Booth, Cameron D. Mackereth, Vivian Saridakis, Irena Ekiel, Guennadi Kozlov, Karen L. Maxwell, Ning Wu, Lawrence P. McIntosh, Kalle Gehring, Michael A. Kennedy, Alan R. Davidson, Emil F. Pai, Mark Gerstein, Structural proteomics of an archaeon. Nature Structural & Molecular Biology. ,vol. 7, pp. 903- 909 ,(2000) , 10.1038/82823
Matthias Eberstadt, Baohua Huang, Zehan Chen, Robert P Meadows, Shi-Chung Ng, Lixin Zheng, Michael J Lenardo, Stephen W Fesik, None, NMR structure and mutagenesis of the FADD (Mort1) death-effector domain. Nature. ,vol. 392, pp. 941- 945 ,(1998) , 10.1038/31972
Sung-Hou Kim, Shining a light on structural genomics. Nature Structural & Molecular Biology. ,vol. 5, pp. 643- 645 ,(1998) , 10.1038/1334
Christopher M. Koth, Maria Victoria Botuyan, Rodney J. Moreland, David B. Jansma, Joan W. Conaway, Ron C. Conaway, Walter J. Chazin, James D. Friesen, Cheryl H. Arrowsmith, Aled M. Edwards, Elongin fromSaccharomyces cerevisiae Journal of Biological Chemistry. ,vol. 275, pp. 11174- 11180 ,(2000) , 10.1074/JBC.275.15.11174
Andrew P. Hansen, Andrew M. Petros, Andrew P. Mazar, Terry M. Pederson, Annemarie Rueter, Stephen W. Fesik, A practical method for uniform isotopic labeling of recombinant proteins in mammalian cells. Biochemistry. ,vol. 31, pp. 12713- 12718 ,(1992) , 10.1021/BI00166A001
Elena Bochkareva, Lori Frappier, Aled M. Edwards, Alexey Bochkarev, The RPA32 Subunit of Human Replication Protein A Contains a Single-stranded DNA-binding Domain Journal of Biological Chemistry. ,vol. 273, pp. 3932- 3936 ,(1998) , 10.1074/JBC.273.7.3932
Richard A. Pfuetzner, Alexey Bochkarev, Lori Frappier, Aled M. Edwards, Replication Protein A Journal of Biological Chemistry. ,vol. 272, pp. 430- 434 ,(1997) , 10.1074/JBC.272.1.430
Takanori Kigawa, Takashi Yabuki, Yasuhiko Yoshida, Michio Tsutsui, Yutaka Ito, Takehiko Shibata, Shigeyuki Yokoyama, CELL-FREE PRODUCTION AND STABLE-ISOTOPE LABELING OF MILLIGRAM QUANTITIES OF PROTEINS FEBS Letters. ,vol. 442, pp. 15- 19 ,(1999) , 10.1016/S0014-5793(98)01620-2