Practical Enzyme Kinetics

摘要: As discussed in chapter 4, the Michaelis-Menten equation (4.10) predicts that a plot of v 0 against S0 is rectangular hyperbola, i.e. one with orthogonal asymptotes, passing through origin (figure 10.1). One limb hyperbola lies completely second quadrant physically meaningless region negative substrate concentration. The arc curve as usually drawn first but this only portion which extends to infinite — υ third quadrant. asymptotes are given by = K m and V. Although S0, relationship simple, its hyperbolic nature gives rise problems presenting kinetic data calculating This because it difficult extrapolate estimate V, asymptotic value 0. In order for approach even within 5 % must be at least 19 times ; apart from possible limitations availability or solubility substrate, interference assay method, high concentrations may result appreciable inhibition (see 4). defined terms V an accurate necessary determining