Determination of nucleic acid base ratios by gas-liquid chromatography
作者： Joseph MacGee
DOI: 10.1016/0003-2697(66)90141-2
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摘要: Abstract A new method for the determination of base ratios nucleic acids has been developed. This uses gas-liquid chromatographic analysis purines and pyrimidines arising on perchloric acid hydrolysis acids. The bases are separated as a single fraction from other products by simple ion-exchange step analyzed methylated derivatives which formed, separated, measured in unit injection alcoholic tetramethylammonium hydroxide. Useful data can be obtained nanomolar levels bases. Refinement techniques should allow extending sensitivity to less than 1 microgram acid.
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sci-hub.se 参考文章(10)
Microdetermination of purines and pyrimidines in biological materials. Journal of Biological Chemistry. ,vol. 189, pp. 597- 605 ,(1951) , 10.1016/S0021-9258(18)44876-4
Treat B. Johnson, Samuel H. Clapp, IX. RESEARCHES ON PYRIMIDINS: SYNTHESES OF SOME NITROGEN-ALKYL DERIVATIVES OF CYTOSIN, THYMIN AND URACIL Thirty-third Paper Journal of Biological Chemistry. ,vol. 5, pp. 49- 70 ,(1908) , 10.1016/S0021-9258(18)91678-9
E. W. Robb, J. J. Westbrook, Preparation of Methyl Esters for Gas Liquid Chromatography of Acids by Pyrolysis of Tetramethylammonium salts. Analytical Chemistry. ,vol. 35, pp. 1644- 1647 ,(1963) , 10.1021/AC60204A029
Terrell C. Myers, Lowell Zeleznick, Alkylation of the Purine Nucleus by Means of Quaternary Ammonium Compounds. I. Tetraalkylammonium Hydroxides Journal of Organic Chemistry. ,vol. 28, pp. 2087- 2089 ,(1963) , 10.1021/JO01043A034
Wilhelm Guschlbauer, Edward G. Richards, Karin Beurling, Alice Adams, Jacques R. Fresco, DETERMINATION OF NUCLEOTIDE COMPOSITION OF POLYRIBONUCLEOTIDES BY SPECTROPHOTOMETRIC ANALYSIS. Biochemistry. ,vol. 4, pp. 964- 975 ,(1965) , 10.1021/BI00881A025
P. Brookes, P. D. Lawley, 254. The methylation of cystosine and cytidine Journal of The Chemical Society (resumed). pp. 1348- 1351 ,(1962) , 10.1039/JR9620001348
R.L. Hancock, D.L. Coleman, GAS CHROMATOGRAPHIC STUDIES ON NUCLEOSIDES. Analytical Biochemistry. ,vol. 10, pp. 365- 367 ,(1965) , 10.1016/0003-2697(65)90280-0
Waldo E. Cohn, The Anion-Exchange Separation of Ribonucleotides Journal of the American Chemical Society. ,vol. 72, pp. 1471- 1478 ,(1950) , 10.1021/JA01160A012
Billy Baggett, Thomas L. Presson, Jack B. Presson, James C. Coffey, CORRECTION FOR QUENCHING OF SAMPLES FROM AN OXYGEN FLASK COMBUSTION METHOD FOR TRITIUM ANALYSIS. Analytical Biochemistry. ,vol. 10, pp. 367- 370 ,(1965) , 10.1016/0003-2697(65)90281-2
H. T. Miles, H. M. Fales, Application of Gas Chromatography to Analysis of Nucleosides. Analytical Chemistry. ,vol. 34, pp. 860- 861 ,(1962) , 10.1021/AC60187A001