Dominance of CD4+ lymphocytic infiltrates with disturbed effector cell characteristics in the tumor microenvironment of prostate carcinoma.

摘要: BACKGROUND: Prostate cancer is the most common of men in Western world. Despite over-expression tumor-associated antigens, like PSA or PSMA, immune activation inefficient. The goal this investigation was to assess situ characteristics prostate cancer-infiltrating lymphocytes and determine their status effector function. METHODS: We compared 17 carcinoma containing tissues, four benign prostatic hyperplasia tissues eight healthy regarding lymphocyte subset composition, locoregional distribution, functional using immunohistological staining cryopreserved tissues. For determination subsets, serial sections were stained with CD3, CD4, CD8 antibodies. Activation function studied CD69, interferon-gamma (IFN gamma), perforin, CD3 zeta chain T-cell-receptor repertoire (TCR) analysis made complexity infiltrating lymphocytes. RESULTS: CD3+, CD4+, CD69+ T prominent derived from patients carcinoma. CD8+ significantly less than CD4+ IFN gamma perforin downregulated on cells tissue. Very few detected within cancerous lesions whereas surrounding showed extensive cluster formation. TCR broad similar that tissue, giving no evidence for specific recruitment. CONCLUSIONS: In microenvironment, dominated while sparse. exhibited signs disturbed Consequently, response against autologous tumor likely be inefficient controlling growth.