Consequence of gastrin-releasing peptide receptor activation in a human colon cancer cell line : A proteomic approach

摘要: Gastrin-releasing peptide (GRP) and its receptor (GRPR) are aberrantly up-regulated in colon cancer. When expressed, they act as morphogens, retaining tumor cells a better differentiated state retarding metastasis. To identify targets activated response to GRPR signaling we studied Caco-2 HT-29 cells, cancer cell lines that expresses function of confluence. Total protein was extracted from pre-confluent (expressing GRP/GRPR) cultured serum-free media the presence or absence GRPR-specific antagonist; well confluent do not express GRPR. Overall, identified 5 proteins specifically down-regulated after GRP/GRPR expression:  Bach2, creatine kinase B, p47, two could be identified; 6 up-regulated:  gephyrin, HSP70, HP1, ICAM-1, ACAT, one identified. These findings suggest mechanism(s) by which mediate morphogenic effects involve act...