A large-tumor-antigen-specific monoclonal antibody inhibits DNA replication of simian virus 40 minichromosomes in an in vitro elongation system.

摘要: In productively infected cells, a fraction of large-tumor antigen (T antigen) is tightly bound to replicating simian virus 40 (SV40) minichromosomes and does not dissociate at salt concentrations greater than 1 M NaCl. We present electronmicrograms demonstrating the presence T on replicated sections SV40 minichromosomes. also show that recognized by antibodies from mouse tumor serum and, more specifically, particular T-antigen-specific monoclonal antibody, PAb 1630. A second 101, react with T-antigen remaining chromatin high concentrations. used an in vitro replication system which allows, via semiconservative DNA replication, completion vivo-initiated replicative intermediate molecules. antibody 1630, but inhibits viral replication. discuss possibility may play role chain elongation during