Extraction of testosterone and epitestosterone in human urine using aqueous two-phase systems of ionic liquid and salt.
作者： Chiyang He , Shehong Li , Huwei Liu , Kean Li , Feng Liu
DOI: 10.1016/J.CHROMA.2005.05.065
关键词:
摘要: Based on aqueous two-phase systems (ATPS) consisting of 1-butyl-3-methylimidazolium chloride, a hydrophilic ionic liquid (IL), and K2HPO4, new simple extraction technique, coupled with reversed-phase high performance chromatography (RP-HPLC), was developed for the simultaneous concentration analysis testosterone (T) epitestosterone (ET) in human urine. Under optimal conditions, efficiencies both analytes were 80-90% one-step extraction. The method required only 3.0 mL urine single hydrolysis/deproteinization/extraction step followed by direct injection IL-rich upper phase into HPLC system analysis. has been satisfactorily applied to T ET detection limits 1 ng/mL linear ranges 10-500 compounds. Compared conventional liquid-liquid or solid extraction, this is much "greener" due no use volatile organic solvent low consumption IL. proposed technique opens up possibilities separation other drugs.
core.ac.uk
elsevier.com
sci-hub.se 参考文章(35)
Per Åke Albertsson, Partition of Cell Particles and Macromolecules ,(1986)
Robin D Rogers, Kenneth R Seddon, Ionic Liquids--Solvents of the Future? Science. ,vol. 302, pp. 792- 793 ,(2003) , 10.1126/SCIENCE.1090313
Jie Ding, Thomas Welton, Daniel W. Armstrong, Chiral ionic liquids as stationary phases in gas chromatography. Analytical Chemistry. ,vol. 76, pp. 6819- 6822 ,(2004) , 10.1021/AC049144C
Jared L. Anderson, Jie Ding, Thomas Welton, Daniel W. Armstrong, Characterizing ionic liquids on the basis of multiple solvation interactions. Journal of the American Chemical Society. ,vol. 124, pp. 14247- 14254 ,(2002) , 10.1021/JA028156H
K.D. Collins, Charge density-dependent strength of hydration and biological structure Biophysical Journal. ,vol. 72, pp. 65- 76 ,(1997) , 10.1016/S0006-3495(97)78647-8
Y Akama, Extraction mechanism of Cr(VI) on the aqueous two-phase system of tetrabutylammonium bromide and (NH4)2SO4 mixture Talanta. ,vol. 57, pp. 681- 686 ,(2002) , 10.1016/S0039-9140(02)00076-0
K. D. Collins, Sticky ions in biological systems Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 5553- 5557 ,(1995) , 10.1073/PNAS.92.12.5553
Huimin Luo, Sheng Dai, Peter V. Bonnesen, A. C. Buchanan, John D. Holbrey, Nicholas J. Bridges, Robin D. Rogers, Extraction of cesium ions from aqueous solutions using calix[4]arene-bis(tert-octylbenzo-crown-6) in ionic liquids Analytical Chemistry. ,vol. 76, pp. 3078- 3083 ,(2004) , 10.1021/AC049949K
Jing-fu Liu, Yu-guang Chi, Gui-bin Jiang, Chao Tai, Jin-feng Peng, Jing-Tian Hu, Ionic liquid-based liquid-phase microextraction, a new sample enrichment procedure for liquid chromatography. Journal of Chromatography A. ,vol. 1026, pp. 143- 147 ,(2004) , 10.1016/J.CHROMA.2003.11.005
Huimin Luo, Sheng Dai, Peter V. Bonnesen, Solvent Extraction of Sr2+ and Cs+ Based on Room-Temperature Ionic Liquids Containing Monoaza-Substituted Crown Ethers Analytical Chemistry. ,vol. 76, pp. 2773- 2779 ,(2004) , 10.1021/AC035473D