Chondrogenic differentiation of mesenchymal stem cells isolated from patients in late adulthood: the optimal conditions of growth factors.

摘要: There is a controversy about the capacity of mesenchymal stem cells (MSCs) from aged individuals to proliferate and differentiate into cartilage. The purpose this study was investigate optimal condition culture human MSCs (>50 years) for cartilage tissue engineering. We tested hypothesis that effective proliferation chondrogenesis can be achieved with under appropriate conditions. To best proliferation, were cultured in medium containing four concentrations subsets (0, 0.05, 0.5, 5 ng/mL) recombinant TGF-beta2 FGF-2, either or without fetal calf serum. cell numbers counted 0, 1, 3, 7 days after growth factors given. For induction 3-dimensional (3-D) culture, pellets chondrogenic combinations various factors. After 4 weeks fixed evaluated Safranin-O staining proteoglycan immunohistochemical type II collagen. RT-PCR also performed mRNAs I collagen, oligomeric protein (COMP). In monolayer 0.5 ng/mL caused significant reduction number irrespective presence FGF-2 most effectively increased even absence pellet remarkable chondrocyte-like differentiation induced around peripheral areas TGF-beta2, accompanied by collagen production. addition 100 IGF-I notable increase contents. results mirrored those histological studies. This shows an may obtained proper individuals.