Meiotic arrest as an alternative to increase the production of bovine embryos by somatic cell nuclear transfer.
作者： F.M.C. Caixeta , R.V. Sousa , A.L. Guimarães , L.O. Leme , J.F.W. Sprícigo
DOI: 10.1017/S0967199416000289
关键词:
摘要: This study aimed to evaluate the effect of meiotic arrest using phosphodiesterase type 3A (PDE 3A) inhibitors, cilostamide and C-type natriuretic peptide (NPPC), on pre-maturation (PM) oocytes be used in production cloned embryos. Nuclear maturation, vitro embryo (IVP), somatic cell nuclear transfer (SCNT) parthenogenetic activation (PA), total cells number embryos were evaluated. The results analysed by chi-squared Kruskal-Wallis test with a P-value 0.05) between control PM, both for cleavage (78.2% 76.9%) blastocyst (35.5% 29.3%) rates. After SCNT, rate was also similar (P > PM (66% 51.9%) however, lower < group than (7.4% 30.2%). 6 h 100 nM NPPC, approximately 84.9% remained at GV. No difference found (69.2% 76.1%) rates (37,4% 35%) after IVP. Similarly, no differences groups observed 68.4%) (24.4% 21.5%) SCNT PA from or had number. It can concluded that NPPC is feasible without affecting outcome.
sci-hub.se 参考文章(43)
G Fecteau, V Bordignon, L C Smith, C Keefer, M Babkine, Benefits and problems with cloning animals. Canadian Veterinary Journal-revue Veterinaire Canadienne. ,vol. 41, pp. 919- 924 ,(2000)
Carol L. Keefer, Artificial cloning of domestic animals Proceedings of the National Academy of Sciences of the United States of America. ,vol. 112, pp. 8874- 8878 ,(2015) , 10.1073/PNAS.1501718112
Heiner Niemann, X Cindy Tian, W Allan King, Rita S F Lee, Epigenetic reprogramming in embryonic and foetal development upon somatic cell nuclear transfer cloning Reproduction. ,vol. 135, pp. 151- 163 ,(2008) , 10.1530/REP-07-0397
M. Zhang, Y.-Q. Su, K. Sugiura, G. Xia, J. J. Eppig, Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes. Science. ,vol. 330, pp. 366- 369 ,(2010) , 10.1126/SCIENCE.1193573
P. Holm, P.J. Booth, M.H. Schmidt, T. Greve, H. Callesen, High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology. ,vol. 52, pp. 683- 700 ,(1999) , 10.1016/S0093-691X(99)00162-4
Jing Chen, Maggie M Chi, Kelle H Moley, Stephen M Downs, cAMP Pulsing of Denuded Mouse Oocytes Increases Meiotic Resumption Via Activation of AMP-activated Protein Kinase Reproduction. ,vol. 138, pp. 759- 770 ,(2009) , 10.1530/REP-08-0535
Federica Franciosi, Giovanni Coticchio, Valentina Lodde, Irene Tessaro, Silvia C. Modina, Rubens Fadini, Mariabeatrice Dal Canto, Mario Mignini Renzini, David F. Albertini, Alberto M. Luciano, Natriuretic Peptide Precursor C Delays Meiotic Resumption and Sustains Gap Junction-Mediated Communication in Bovine Cumulus-Enclosed Oocytes Biology of Reproduction. ,vol. 91, pp. 61- ,(2014) , 10.1095/BIOLREPROD.114.118869
M.A.N Dode, P.R Adona, Developmental capacity of Bos indicus oocytes after inhibition of meiotic resuption by 6-dimethylaminopurine. Animal Reproduction Science. ,vol. 65, pp. 171- 180 ,(2001) , 10.1016/S0378-4320(00)00207-4
P.J.M. Hendriksen, P.L.A.M. Vos, W.N.M. Steenweg, M.M. Bevers, S.J. Dieleman, Bovine follicular development and its effect on the in vitro competence of oocytes Theriogenology. ,vol. 53, pp. 11- 20 ,(2000) , 10.1016/S0093-691X(99)00236-8
Keith E. Latham, Early and delayed aspects of nuclear reprogramming during cloning. Biology of the Cell. ,vol. 97, pp. 119- 132 ,(2005) , 10.1042/BC20040068