Amino Acid Incorporation and Aminoacyl Transfer in an Insect Pupal System

摘要: An amino acid-incorporating system which has very high endogenous activity been obtained from insect pupae. It is similar to those other sources in that it requires microsomal particles, transfer ribonucleic acid, adenosine triphosphate, guanosine a nucleoside triphosphate-regenerating system, and magnesium ions. Supernatant fluid Escherichia coli or rabbit liver can stimulate the 5-fold because of supply aminoacyl acid. The requirement for concentration K+ be abolished by addition GTP-regenerating system. In potassium, partial inhibition GTPase was observed. Incorporation leucine leucyl acid ribosomes, Mg++, GTP. Puromycin ribonuclease cause almost complete incorporation. complementary enzymes incorporation free acids into polypeptide aminoacyl-tRNA protein are bound tightly microsomes. These stripped microsomes with KCl. When ribosomes 1st day pupae were incubated enzyme tRNA prepared 7th pupae, increase ratio tryosine observed, suggesting control cuticular synthesis at translation level.