Next generation sequencing of sex-specific genes in the livers of obese ZSF1 rats.
作者： Andrea Babelova , Birgitta C. Burckhardt , Gabriela Salinas-Riester , Claudia Pommerenke , Gerhard Burckhardt
DOI: 10.1016/J.YGENO.2015.07.006
关键词:
摘要: Type 2 diabetes induces pathophysiological changes in the liver. The aim of this study was to identify differently expressed genes livers male and female ZSF1 rats (ZDFxSHHF-hybrid, generation F1), a model for type diabetes. Gene expression investigated using next-generation sequencing (NGS). Selected candidate were verified by real-time PCR obese lean rats. 103 sex-different genes, associated pathways "response chemical stimulus", "lipid metabolism", organic substance", identified. Male-specific involved hepatic metabolism, detoxification, secretion, e.g. cytochrome P450 2c11 (Cyp2c11), Cyp4a2, glutathione S-transferases mu (Gstm2), Slc22a8 (organic anion transporter 3, Oat3). Most female-specific lipid metabolism (e.g. glycerol-3-phosphate acyltransferase 1, Gpam) or glycolysis glucokinase, Gck). Our data suggest necessity pay attention sex- diabetes-dependent pre-clinical testing metabolized secreted drugs.
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