Growth parameters of Borrelia burgdorferi sensu stricto at various temperatures
作者： M. Heroldová , M. Němec , Z. Hubálek
DOI: 10.1016/S0934-8840(98)80058-3
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摘要: Summary Growth of Borrelia burgdorferi sensu stricto (prototype strain B-31) was studied in Barbour-Stoenner-Kelly BSK-H liquid medium, supplemented with 4.5 % rabbit serum and antibiotics (phosphomycin, rifampicin), at various temperatures to early stationary growth phase. The number cells determined by darkfield microscopy. In the range cultivation 25 °C 37 °C, generation time between 8.26 12.36 h (the shortest one 33 °C), specific rate 0.056 0.083 h-1 highest °C). optimum temperature for B. although good also observed 28 30 35 37°C. grew well but slowly whereas no 20 °C.
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