Differentiation and Phylogeny of Trichogrammatidae (Hymenoptera: Chacidoidea) from Mexico Based on ITS2 and 18S Molecular Markers of rDNAr and COII of the mDNA

作者: Verónica Ávila-Rodríguez , Omar G. Alvarado-Gómez , Alejandro González-Hernández , Urbano Nava-Camberos

DOI: 10.3958/059.038.0213

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摘要: Abstract. A great diversity of trichogrammatids (35 genera) has been reported for Mexico. Particularly, several species the genus Trichogramme are widely used as biological control agents in integrated agricultural pest management programs The identification trichogrammatid wasps based on morphological characters is complicated. feasible alternative to those taxonomic problems utilization molecular techniques. Therefore, objectives present study were differentiate most common genera Trichogrammatidae, and infer their phylogeny, markers ITS2 18S rDNA COII mDNA. Thus, individual Ittys, Aphelinoidea, Paracentrobia, Pseudoligosita, Ufens, Burksiella, Zagella, Trichogramma subjected DNA extraction, amplification by PCR, phylogenetic analysis maximum parsimony (MP) method, use ribosomal (rDNA) COM mitochondrial (mD...

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