A role for the Fas/Fas ligand apoptotic pathway in regulating myeloid progenitor cell kinetics

摘要: Abstract Objective Bone marrow from wild-type mice and with mutated Fas ( lpr ) or ligand gld was used to investigate the role of Fas/FasL system in regulation myeloid progenitor cell kinetics. Methods Granulocyte-macrophage colony-forming cells (CFU-GM) were measured by a standard colony assay proliferative activity CFU-GM replating primary colonies observing secondary formation. expression restored mouse bone retrovirus-mediated gene transfer treated soluble FasL. Wild-type YVAD (a caspase inhibitor) anti-Fas monoclonal antibodies. Results There greater frequencies compared (WT) (p = 0.0008). The capacity also significantly for WT 0.0003 0.0001, respectively). Retrovirus-mediated restoration into marrow, provision FasL (sFasL) reduced proliferation levels. Treatment anti-FasL antibody increased levels found CFU-GM. human 0.015 0.04, Conclusion Fas, FasL, activation may play an important regulating