MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice.

摘要: MicroRNA (miRNA) regulates gene expression in a post-transcriptional manner, which hybridizes the target mRNAs with complementary sequence and subsequently leads to translation repression or mRNA degradation. Online alignment showed that there is putative binding site of miR-223-3p on 3'UTR LIF, considered be an important marker endometrial receptivity. Thus, we hypothesized may affect embryo implantation by suppressing LIF expression. In this study, found protein was inversely expressed endometrium mice during window. Then proved directly binds luciferase reporter assay supresses LIF. To investigate whether miR-233-3p affects implantation, agonist injected into uteri pregnant mice. The results demonstrated effect implantation. Furthermore, over-expression compromise pinopodes formation Taken all together, our findings revealed suppresses expression, lead diminished